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隋广超

 

联系方式:

E-mail:  gcsui@nefu.edu.cn

电话:    86-451-8219-1081

地址:    黑龙江省哈尔滨市香坊区和兴路26号

东北林业大学生命科学学院 (新逸夫教学楼) 117 房间

教育和训练经历:

1983/09-1987/07,北京大学,生物系,生物化学专业学士

1990/10-1995/07,北京大学,生命科学学院,生物化学及分子生物学博士

1995/10-1998/11,瑞典 Karolinska 医学院,临床化学系,医学博士

1998/11-1999/07,瑞典 Karolinska 医学院,临床化学系,博士后

1999/07-2004/08,美国哈佛大学哈佛医学院,病理系,博士后

工作经历:

2004/09-2005/08,美国哈佛大学哈佛医学院,讲师

2005/09-2012/09,美国Wake Forest 大学医学院癌症生物学系,美国NIH 综合癌症研究中心,助教授

2012/10-2014/06,美国Wake Forest 大学医学院癌症生物学系,美国NIH 综合癌症研究中心,副教授

2014/07-现在,美国Wake Forest大学医学院癌症生物学系co-faculty (无工资和工作时间要求)

2014/01-现在,东北林业大学生命科学学院,教授 (全职)

专业特长:

分子生物学,生物化学,细胞生物学,遗传学及表观遗传学,非编码RNA (noncoding RNA),基因表达及调控,癌症生物学。

研究经验和研究领域:

我的实验室所涉及到的研究方法及内容是:利用分子生物学、细胞生物学和动物模型来研究癌症的发生与发展过程中的分子调控机制。我具有多年的在癌症研究领域内的实践经验,在本领域内的高影响因子刊物上,以第一作者或通讯作者发表了50余篇科研论文。作为项目主持人领导过包括美国NIH,ACS和DOD资助的多项重大研究课题。在美国期间,曾经十几次被多个癌症研究基金会聘请为基金评审组的专家评委,并担任多个科研刊物的评委或编辑。

我对研究领域的贡献可以归纳为以下几个方面:

1)发明了以DNA载体为基础的RNA干扰(RNAi)技术。这一技术的发明为许多生物学科的研究提供了全新的方法,尤其是对基因和蛋白质功能的研究具有重大的创新性。它简化了以往的化学合成小干扰RNA(siRNA)的方法,并使得在细胞内稳定表达siRNA以达到长期抑制靶基因表达成为可能,也促进了RNAi技术在医疗上的应用。目前,这一方法已经成为世界范围实验室用于研究基因功能的常规实验技术。这一方法DNA载体已成为美国Ambion/Life Technologies公司的第一代产品(商品名pSilencer)。做为第一作者发表这一方法的PNAS文章已经被引用了上千次。

2)利用对Yin Yang 1(YY1)蛋白在肿瘤发生中功能的研究来探索几种常见癌症(乳腺癌,前列腺癌,肺癌)的致病机制。我的多年的研究成果确立了YY1的癌基因地位。作为一个多功能蛋白,YY1在癌症发生中的终极作用一直有争议。我在2004年发表的Cell文章中发现了YY1对p53的负调控,奠定了YY1的癌基因的地位。在2005年后自己领导的实验室内,做为通讯作者发表了15篇YY1相关的论文。这些研究进一步确定YY1的癌基因地位,并揭示了YY1在癌症治疗中作为靶基因的可能性。

3)对其它抑癌基因、原癌基因及非编码RNA的研究:(1)利用基因芯片等方法对抑癌基因SOX7的研究,发现其上、下游调控因子及可能的调节机制;(2)对Dmp1 研究,发现了这一基因产生mRNA的不同选择性拼接(alternative splicing)形态对乳腺癌发生和发展具有完全相反的作用;(3)研究了几种microRNA对原癌基因Ezh2、YY1和FASN的调节作用;(4)利用多个乳腺癌及它们对应的癌旁组织样品中的长非编码RNA(lncRNA)的分析,发现了多种在乳腺癌中差异性表达的lncRNA,目前正在对它们的生物学意义和对乳腺癌的调节功能进行鉴定。

教学情况:

曾经在Wake Forest医学院教授: Oncogenes, Tumor suppressors, Cancer Epigenetics, Non-coding RNAs.  将开设课程:表观遗传学与应用

基金评审经历 (专家评委, Scientist Reviewer):

美国国防部 (DOD) 癌症研究基金评委 (2007-2014; 11次)

美国癌症协会 (ACS) 研究基金评委 (2013)

意大利健康部 (2010, 2013, 2015)

英国研究基金 (2012)

近年承担的主要课题:

国家自然科学基金 (项目主持人): 2015年01月至 2018年12月

题目: SOX7在乳腺癌中调控机制的研究

黑龙江省自然科学基金重点项目 (项目主持人): 2015年07月至 2018年06月

题目: 利用YY1的OPB肽段抑制肿瘤细胞生长

美国National Institute of Health (NIH): R01, 2009 - 2014

项目主持人 (PI): Guangchao Sui

题目: Dmp1-p53/YY1 interplays and their roles in lung cancer development

美国American Cancer Society (ACS):Research Scholar Grant, 2009 - 2013

项目主持人 (PI): Guangchao Sui

2009 – 2013:The role of YY1 in prostate cancer

其它承担过的基金 (项目主持人: Guangchao Sui):

美国Department of Defense (DOD)

Golfers Against Cancer (GAC)

Kulynych Interdisciplinary Cancer Research Fund

Cell Growth and Survival Program Push Grant

主要发表文章: (* 为通讯作者)

1.       Guangchao Sui, Christina Soohoo, El Bachir Affar, Frederique Gay, Yujiang Shi, William C. Forrester, and Yang Shi. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. (2002) Proc. Natl. Acad. Sci. USA, 99:5515-5520. http://dx.doi.org/doi:10.1073/pnas.082117599

2.       Yujiang Shi, Junichi Sawada, Guangchao Sui, El Bachir Affar, Johnathan R. Whetstine, Fei Lan, Hidesato Ogawa, Margaret Po-Shan Luke, Yoshihiro Nakatani and Yang Shi. Coordinated histone modifications mediated by a CtBP co-repressor complex. (2003) Nature, 422:735-738. http://dx.doi.org/doi:10.1038/nature01550

3.       Guangchao Sui, El Bachir Affar, Yujiang Shi, Chrystelle Brignone, Nathan R. Wall, Peng Yin, Mary Donohoe, Margaret P. Luke, Dominica Calvo, Steven R. Grossman and Yang Shi. Yin Yang 1 is a negative regulator of p53. Cell, 2004;117:859-872.

http://dx.doi.org/doi:10.1016/j.cell.2004.06.004

4.       Peter Baumeister, Shengzhan Luo, William C. Skarnes, Guangchao Sui, Edward Seto, Yang Shi and Amy S. Lee. Endoplasmic reticulum stress induction of the Grp78/BiP promoter: activating mechanisms mediated by YY1 and its interactive chromatin modifiers. (2005) Mol. Cell. Biol., 25:4529-4540. http://dx.doi.org/doi:10.1128/MCB.25.11.4529-4540.2005

5.       Guangchao Sui and Yang Shi. Gene silencing by a DNA vector-based RNAi technology. (2005) Methods Mol. Biol., 309:205-218. http://dx.doi.org/doi:10.1385/1-59259-935-4:205

6.       El Bachir Affar, Margaret Po-shan Luke, Frederique Gay, Dominica, Calvo, Guangchao Sui, Robert S. Weiss, En Li, and Yang Shi. Targeted ablation of Par-4 reveals a cell type-specific susceptibility to apoptosis-inducing agents. (2006) Cancer Research. 66:3456-3462. http://dx.doi.org/doi:10.1158/0008-5472.CAN-05-0964

7.       Margaret P. Luke, Guangchao Sui, Huifei Liu and Yang Shi. Yin Yang 1 physically interacts with HoxA11 and represses HoxA11-depedent transcription. (2006) J. Biol. Chem., 281:33226-33232. http://dx.doi.org/doi:10.1074/jbc.M606584200

8.       Zhiyong Deng, Meimei Wan and Guangchao Sui*. PIASy-mediated sumoylation of Yin Yang 1 depends on their interaction but not the RING finger. (2007) Mol. Cell. Biol., 27(10):3780-3792. http://dx.doi.org/doi:10.1128/MCB.01761-06

9.       Dohoon Kim, Minh Nguyen, Matthew Dobbin, Andre Fischer, Farahnaz Sananbenesi, Joseph Rodgers, Ivana Delalle, Joseph Baur, Guangchao Sui, Sean M Armour, Pere Puigserver, David Sinclair and Li-Huei Tsai. SIRT1 deacetylase protects against neurodegeneration in models for Alzheimer's disease and amyotrophic lateral sclerosis. (2007) EMBO J. 26(13):3169–3179.

http://dx.doi.org/doi:10.1038/sj.emboj.7601758

10.   Zhiyong Deng, Meimei Wan, Paul Cao, Anuradha Rao, Scott D. Cramer and Guangchao Sui*. Yin Yang 1 Regulates the Transcriptional Activity of Androgen Receptor. (2009) Oncogene; 28:3746-3757. http://dx.doi.org/doi:10.1038/onc.2009.231

11.   Guangchao Sui*.The regulation of YY1 in tumorigenesis and its targeting potential in cancer therapy. (2009) Mol. Cell. Pharmacol. 1(3):157-176. (Invited review) 

      http://dx.doi.org/DOI:10.4255/mcpharmacol.09.20

12.   Zhiyong Deng, Paul Cao, Meimei Wan and Guangchao Sui*. Yin Yang 1: a multifaceted protein beyond a transcription factor. (2010) Transcription. 1(2):81-84. (Invited review) http://dx.doi.org/10.4161/trns.1.2.12375

13.   Paul Cao, Zhiyong Deng, Meimei Wan, Weiwei Huang, Scott D Cramer, Jianfeng Xu, Ming Lei and Guangchao Sui*. MicroRNA-101 Negatively Regulates Ezh2 and Its Expression is Modulated by Androgen Receptor and HIF-1alpha/HIF-1beta. (2010) Mol. Cancer, 9(1):108. http://dx.doi.org/doi:10.1186/1476-4598-9-108

14.   Linara S. Axanova, Yong Q. Chen, Thomas McCoy, Guangchao Sui and Scott D. Cramer. 1,25-dihydroxyvitamin D3 and PI3K/AKT inhibitors synergistically inhibit growth and induce senescence in prostate cancer cells. (2010) Prostate. 70(15):1658-1671. http://dx.doi.org/doi:10.1002/pros.21201

15.   Rui Fang, Andrew J. Barbera, Yufei Xu, Michael Rutenberg, Thiago Leonor, Qing Bi, Fei Lan, Pinchao Mei, Guo-Cheng Yuan, Christine Lian, Junmin Peng, Dongmei Cheng, Guangchao Sui, Ursula B. Kaiser, Yang Shi and Yujiang Geno Shi. Human LSD2/KDM1b/AOF1 Regulates Gene Transcription by Modulating Intragenic H3K4me2 Methylation. (2010) Mol. Cell, 39(2):222-233.

http://dx.doi.org/doi:10.1016/j.molcel.2010.07.008

16.   Zandra K. Pinnix, Lance D. Miller, Wei Wang, Ralph D’Agostino Jr., Tim Kute, Mark C. Willingham, Heather Hatcher, Lia Tesfay, Guangchao Sui, Xiumin Di, Suzy V. Torti, Frank M. Torti. Ferroportin and Iron Regulation in Breast Cancer Progression and Prognosis (2012) Sci Transl Med. 22, 43ra56.http://dx.doi.org/doi:10.1126/scitranslmed.3001127

17.   Helen Yu, Nazar Mashtalir, Salima Daou, Ian Hammond-Martel, Julie Ross, Guangchao Sui, Gerald W. Hart, Frank J. Rauscher III, Elliot Drobetsky, Eric Milot, Yang Shi and El Bachir Affar. The Ubiquitin Carboxyl Hydrolase BAP1 Forms a Ternary Complex with YY1 and HCF-1 and is a Critical Regulator of Gene Expression. (2010) Mol. Cell. Biol., 30(21):5071-5085.

http://dx.doi.org/doi:10.1128/MCB.00396-10

18.   Salima Daou, Nazar Mashtalir, Ian H Martel, Helen Pak, Helen Yu, Guangchao Sui, Jodi Vogel, Thomas Kristie, El Bachir Affar. Crosstalk Between O-GlcNAcylation And Proteolytic Cleavage Regulates The Host Cell Factor-1 Maturation Pathway. (2011) Proc. Natl. Acad. Sci. USA, 108(7):2747-2752.http://dx.doi.org/doi:10.1073/pnas.1013822108

19.   Sophia L Maund, Wendy W Barclay, Laura D Hover, Linara S Axanova, Guangchao Sui, Jason D Hipp, James C. Fleet, Andrew Thorburn, and Scott D Cramer. Interleukin-1α Mediates the Antiproliferative Effects of 1,25-Dihydroxyvitamin D3 in Prostate Progenitor/Stem Cells. (2011) Cancer Research, 2011;71(15):5276-5286.

http://dx.doi.org/doi:10.1158/0008-5472.CAN-10-2160

20.   Qiang Zhang, Daniel Stovall, Kazushi Inoue and Guangchao Sui*. The oncogenic role of Yin Yang 1. Critical Reviews in Oncogenesis, 2011;16(3-4):163-197. (Invited review) http://dx.doi.org/DOI:10.1615/CritRevOncog.v16.i3-4.30

21.   Weiwei Huang, Philip J. Smaldino, Qiang Zhang, Lance D. Miller, Paul Cao, Kristin Stadelman, Meimei Wan, Banabihari Giri, Ming Lei, Yoshikuni Nagamine, James P. Vaughn, Steven A. Akman and Guangchao Sui*. Yin Yang 1 contains G-quadruplex structures in its promoter and 5’-UTR and its expression is modulated by G4 Resolvase 1. (2012) Nucleic Acids Research, 40(3):1033-1049.http://dx.doi.org/doi:10.1093/nar/gkr849

22.   Shihua Wang, Jiansheng Wu, Janel Suburu, Zhennan Gu, Jiaozhong Cai, Linara S. Axanova, Scott D. Cramer, Michael J. Thomas, Donna L. Perry, Iris Edwards, Lorelei Mucci, Jennifer Sinnott, Massimo Loda, Guangchao Sui, Isabelle M. Berquin and Yong Q. Chen. Effect of Dietary Polyunsaturated Fatty Acids on Castration-resistant Pten-null Prostate Cancer. (2012) Carcinogenesis, 33(2):404-412.http://dx.doi.org/doi:10.1093/carcin/bgr290

23.   Wennuan Liu, Johan Lindberg, Guangchao Sui, Jun Luo, Tao Li, Carol Xie, Meimei Wan, Seong-Tae Kim, Aubrey Turner, Jishan Sun, Yi Zhu, Zheng Zhang, Scott D Cramer, Robert L. Vessella, S. Lilly Zheng, Henrik Grönberg, William B. Isaacs and Jianfeng Xu. Genome-wide analysis of somatic DNA copy number reveals novel CHD1-associated collaborative alterations of genomic structure in prostate cancer. (2012) Oncogene, 31(35):3939-48.

http://dx.doi.org/doi:10.1038/onc.2011.554  

24.   Caroline I. Schnegg, Mitra Kooshki, Fang-Chi Hsu, Guangchao Sui and Mike E. Robbins. PPARδ prevents radiation-induced proinflammatory responses in microglia via transrepression of NF-кB and inhibition of the PKCα/MEK1/2/ERK1/2/AP-1 pathway. (2012) Free Radical Biology and Medicine, 52:1734-1743.http://dx.doi.org/10.1016/j.freeradbiomed.2012.02.032

25.   Donna P. Frazier, Robert D. Kendig, Fumitake Kai, Dejan Maglic, Takayuki, Sugiyama, Rachel L. Morgan, Sarah J. Lagedrost, Guangchao Sui and Kazushi Inoue. Dmp1 physically interacts with p53 and positively regulates p53’s stability, nuclear localization and transcriptional activity. (2012) Cancer Research, 72:1740-1750.http://dx.doi.org/doi:10.1158/0008-5472.CAN-11-2410

26.   Meimei Wan, Weiwei Huang, Timothy E. Kute, Lance Miller, Qiang Zhang, Heather Hatcher, Jingxuan Wang, Daniel Stovall, Gregory B. Russell, Paul Cao, Zhiyong Deng, Wei Wang, Qingyuan Zhang, Ming Lei, Suzy Torti, Steven A. Akman and Guangchao Sui*. Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27. (2012) American Journal of Pathology, 2012;180(5):2120-2133.http://dx.doi.org/10.1016/j.ajpath.2012.01.037

27.   Min Wu, Lihong Shi, Adela Cimic, Lina Romero, Guangchao Sui, Cynthia J. Lees, J. Mark Cline, Darren F. Seals, Joseph S. Sirintrapun, Thomas P. McCoy, Jielin Sun, Seong-Tae Kim, Wennuan Liu, Donna M. Peehl, Jianfeng Xu, Scott D. Cramer. Suppression of Tak1 promotes prostate tumorigenesis. (2012) Cancer Research, 72(11):2833-2843.  http://dx.doi.org/doi:10.1158/0008-5472.CAN-11-2724

28.   Shuai Tang, Meenu Mishra, Donna P. Frazier, Miranda L. Moore, Kazushi Inoue, Rajendar Deora, Guangchao Sui and Purnima Dubey. Positive and negative regulation of prostate stem cell antigen expression by Yin Yang 1 in prostate epithelial cell lines. (2012) PLoS One, 7(4): e35570.

http://dx.doi.org/doi:10.1371/journal.pone.0035570

29.   Daniel Stovall and Guangchao Sui*. The Function of YY1 and its Oncogenic Role in Prostate Cancer. In: Gerhard Hamilton, eds. Advances in Prostate Cancer. InTech. 2012:563-587.  http://dx.doi.org/10.5772/53091

30.   Daniel Stovall, Meimei Wan, Qiang Zhang, Purnima Dubey and Guangchao Sui*. DNA Vector-based RNA Interference to Study Gene Function in Cancer. (2012) Journal of Visualized Experiments, (64), pii: 4129. http://dx.doi.org/doi:10.3791/4129

31.   Zhiyong Deng, Guangchao Sui, Paulo Mottin Rosa and Weiling Zhao. Radiation-Induced c-Jun Activation Depends on MEK1-ERK1/2 Signaling Pathway in Microglial Cells. (2012) PLoS One, 7(5):e36739. http://dx.doi.org/doi:10.1371/journal.pone.0036739

32.   Wei Wang, David Grier, Jennifer Woo, Martha Ward, Guangchao Sui, Suzy V. Torti, Frank M. Torti, and Michael W. Beaty. Ferritin H is a novel marker of early erythroid precursors and macrophages. (2013) Histopathology. 62(6):931-40.http://dx.doi.org/ doi:10.1111/his.12101

33.   Sinan Zhu, Ryan T. Mott, Elizabeth A. Fry, Pankaj Taneja, George Kulik, Guangchao Sui*, and Kazushi Inoue. Cooperation between Dmp1-loss and cyclin D1 overexpression in breast cancer. (2013) American Journal of Pathology. 183(4):1339-1350 http://dx.doi.org/doi:10.1016/j.ajpath.2013.06.027

34.   Daniel B. Stovall, Meimei Wan, Lance D. Miller, Paul Cao, Dejan Maglic, Qiang Zhang, Martha R. Stampfer, Wennuan Liu, Jianfeng Xu and Guangchao Sui*. The Regulation of SOX7 and its Tumor Suppressive Role in Breast Cancer. (2013) American Journal of Pathology 183(5):1645-1653. http://dx.doi.org/doi:10.1016/j.ajpath.2013.07.025

35.   Dana Yancey, Kyle C. Nelson, Daniele Baiz, Sazzad Hassan, Anabel Flores, Ashok Pullikuth, Yelena Karpova, Victoria Moore1, Guangchao Sui and George Kulik. BAD Dephosphorylation and Decreased Expression of MCL-1 Induce Rapid Apoptosis in Prostate Cancer Cells. (2013) PLoS One. 8(9):e74561. http://dx.doi.org/doi:10.1371/journal.pone.0074561

36.   Daniele Baiz, Sazzad Hassan, Young A Choi, Anabel Flores, Yelena Karpova, Dana Yancey, Ashok Pullikuth, Guangchao Sui, Michel Sadelain, Waldemar Debinski and George Kulik. Combination of the PI3K Inhibitor ZSTK474 with a PSMA-Targeted Immunotoxin Accelerates Apoptosis and Regression of Prostate Cancer. (2013) Neoplasia. 15(10):1720-1183.  http://dx.doi.org/doi:10.1593/neo.13986

37.   Elizabeth A. Fry, Pankaj Taneja, Dejan Maglic, Sinan Zhu, Guangchao Sui*, and Kazushi Inoue. Dmp1α Inhibits HER2/neu-induced Mammary Tumorigenesis. (2013) PLoS One. 8(10):e77870.  http://dx.doi.org/doi:10.1371/journal.pone.0077870

38.   Guorui Deng and Guangchao Sui*. Noncoding RNA in Oncogenesis: A New Era of Identifying Key Players. (2013) Int. J. Mol. Sci. 14:18319-18349. (Invited review)

http://dx.doi.org/doi:10.3390/ijms140918319

39.   Wei Wang, Zhiyong Deng, Heather Hatcher, Lance D. Miller, Xiumin Di, Lia Tesfay, Guangchao Sui, Ralph B. D'Agostino Jr., Frank M. Torti, and Suzy V. Torti. IRP2 regulates breast tumor growth. (2014) Cancer Research. 74(2):497-507.

http://dx.doi.org/doi:10.1158/0008-5472.CAN-13-1224

40.   Nicholas R. Galloway, Carlos J. Diaz Osterman, Karl Reiber, Jessica M.S. Jutzy, Fengzhi Li, Guangchao Sui, Ubaldo Soto, Nathan R. Wall. Yin Yang 1 regulates the transcriptional repression of surviving. (2014) Biochem Biophys Res Commun. 445(1):208-213. http://dx.doi.org/doi:10.1016/j.bbrc.2014.01.169

41.   Daniel B. Stovall, Paul Cao and Guangchao Sui*. SOX7: From a developmental regulator to an emerging tumor suppressor. (2014) Histol Histopathol. 29(4):439-445. http://www.hh.um.es/Abstracts/Vol_29/29_4/29_4_439.htm

42.      Dejan Maglic, Daniel B. Stovall, Mark Cline, Elizabeth A. Fry, Ali Mallakin, Pankaj Taneja, David L. Caudell, Mark C. Willingham, Guangchao Sui*, and Kazushi Inoue. DMP1β, a splice isoform of the tumor suppressor DMP1 locus, induces proliferation and progression of breast cancer. (2015) J Pathol. (In print).http://dx.doi.org/doi:10.1002/path.4504

43.    Jinming Shi, Aixin Hao, Qiang Zhang, and Guangchao Sui*. The Role of YY1 in Oncogenesis and Its Potential as a Drug Target in Cancer Therapies. (2015) Current Cancer Drug Targets. 15(2):145-157.        http://dx.doi.org/10.2174/1568009615666150131124200.

44.     Qiang Zhang, Meimei Wan, Jinming Shi, David A. Horita, Lance D. Miller, Timothy E. Kute, Steven J. Kridel, George Kulik, and Guangchao Sui*. Yin Yang 1 Promotes mTORC2-mediated AKT Phosphorylation. (2015) J Mol Cell Biol. (Accepted).

发明专利:

Yang ShiandGuangchao Sui. Compositions and Methods of Suppressing Eukaryotic Gene Expression. Patent Application 10/301,516, Harvard Case 2032

 

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